Sealed nib waters xbridge protein beh c4 column 300a 3. Develop robust methods across the entire ph spectrum using an unparalleled range of mobile phases and temperatures. Xbridge peptide beh c, care and use manual read online or download pdf waters xbridge peptide beh c18 a and 300a columns user manual manuals directory online owner manuals library. Xbridge columns include a selection of 10 generalpurpose and applicationspecific sorbents that cover a wide range of particles sizes for analytical and preparative hplc applications. The xbridge c18 packing materials were designed to provide excellent peak shape, high efficiency, and excellent stability for acidic and basic mobile phases. It is important to ensure mobile phase compatibility before changing to a different mobile phase system. Note that most columns will have shorter column lifetime when. The certificate of analysis is specific to the batch of packing material contained in the xbridge oligonucleotide c 18. This listing is for a waters xbridge peptide beh c18 prep column, a, 5um, 10mm x 100mm.
Figure 7 demonstrates eclipse xdbcn has superior stability at mid ph when compared to other cn bonded phases. What is the difference between xbridge c18, symmetry c18, and sunfire c18. Recommended ph and temperature limits for xbridge columns at ambient temperatures name of column particle size pore diameter surface area ph limits temperature limits surface carbon load low ph high ph xbridge c18 2. What is a good column choice for the analysis of vitamin d3. C18 modified silica material without polar embedded groups, in this study xbridge beh c18 3. What is the difference between xbridge beh c18 and xbridge.
Xbridge beh c18 xp column waters running alternately with an acidic or a basic mobile phase. What is the recommended storage solvent for the xbridge. The two most important aspects of method transfer after scaling the gradient to the new column dimensions and particle size are 1 system volumes of the lc system and uplc and the 2 temperature. Where can i find the care and use manual for xbridge columns. Feb 15, 2020 waters manufacturer usa introduced three different types of c18 stationary phases, namely, the xbridge c18, the xbridge shield rp18 and the xselect csh c18. This listing is for a waters xbridge peptide beh c18 column, 300a, 3. What is a good column to separate peptides from metalloid. Under accelerated lowph test conditions, xbridge c18 columns show little. Dec 21, 2015 care and use manual xbridge columns 4 table 2. This standard should be used on a regular basis for evaluation of column and system performance. Agilent zorbax eclipse xdb columns c18, c8, phenyl and cn provide four bonded phase choices for method development optimization. Care and use manual waters xbridge peptide beh c18 care and use manual 11 pages a and 300a columns.
Ethylene bridged hybrid beh columns lead the industry. Waters xbridge peptide beh c18 a and 300a columns manual. How do i clean an acquity beh amide or xbridge amide. Oct 02, 2020 for additional information see xbridge peptide beh c18 a and 300a columns care and use manual, xbridge protein beh, c4, 300a, 3. Xbridge hplc and uhplc columns, utilizing beh particle technology, are built to withstand high ph, high temperatures, and other extreme operating conditions, including volatile eluents, for a wide range of small molecule and biomolecule lcms analysis. The xbridge column family is designed for maximum method development flexibility. The cleaning and regeneration of reversedphase hplc columns. Xbridge peptide beh c18 medical equipment pdf manual download. The flow rates given in the procedure below are for a typical 2.
Hplc column equivalent xbridge beh c 18 xbridge beh shield rp18 xbridge beh c 8 xbridge beh phenyl xbridge beh hilic xbridge beh amide hplc particle sizes 2. The xbridge packing materials were designed to provide excellent peak shape, high efficiency, and excellent stability for acidic and basic mobile phases. Aeris widepore xb c18 and xbc8 columns were designed to operate at column temperatures up to 90c. The dual column uhplc platform was hyphenated to a tripletof 5600 ms ab sciex. Jun 29, 2016 2017 waters corporation 5company confidential industry leading waters oligonucleotide columns with beh technology acquity uplc columns for optimum speed, performance and throughput beh, c18, a, 1. Aeris peptide xb c18 columns can be used up to 90c.
Hplc guard cartridge xbridge beh c18 a 5 m 30 mm x 10 mm. Xbridge column users can quickly develop robust methods using mobile phases throughout the entire ph range of 112. The organic fractions were evaporated to dryness, reconstituted and injected onto a 150 x 2. Aeris widepore c4 columns can be operated up to 60c. Part number pack size uom description type id length. The atlantis dc18 columns feature difunctionally bonded c18 ligands that have been optimized for use with highly aqueous mobile. To maintain maximum column performance, use high quality chromatography grade solvents.
Xbridge peptide beh c18, a and 300a columns and xbridge. If you are looking for the care and use manual for 186006729 xselect csh c18 2. Xbridge columns care and use manual waters corporation. These columns are designed with the ethylene bridged hybrid beh, the optimum bed density obd and the charge surface hybrid csh technologies. Where can i find the care and use manual for xbridge. Equilibrate the column with a minimum of 10 column volumes of the mobile phase to be used refer to t able 1 for a list of column volumes. What is the storage solvent for xbridge reversephase columns. Flush column with 100% organic mobile phase methanol or acetonitrile by setting the pump flow rate to 0. Here is a new sealed waters xbridge beh c18 column, a, 2. Each xbridge peptide and xbridge premier peptide beh.
All pictures shown are for illustration purpose only. The attached manual describes about xbridge peptide beh c18, a and 300a columns and xbridge premier peptide beh c18, a columns and. The massprep oligonucleotide standard is designed for verification of hplcuplc instruments and performance of columns used for analysis of synthetic oligonucleotides, specifically the acquity and xbridge beh and 300 c18 columns. Waters xbridge peptide beh c18 care and use manual pdf.
For isolation and purification, the high ph stability of xbridge prep obd columns allows for purer fractions and increased mass loading for bases. The system volumes entered into the calculator must be accurate. Browse online or download waters xbridge peptide beh c18 a and 300a columns user manual 9 pages. Xbridge columns 1 thank you for choosing a waters xbridge column.
Waters xbridge peptide beh c18 prep column, a, 5um. As a workhorse for hplc method development, xbridge beh c18 sorbent can be used over an entire range of mobile phase ph 112. Xpbridge amide columns are effective for the separation of highly polar, charged metabolites in hilic mode. Waters xbridge columns xbridge beh c18 column 186003117.
Long column life with all bonded phases c18 and c8 columns are known for their long lifetimes. Owners manuals and users guides for water equipment waters xbridge columns. An online 2dreversedphase reversedphase chromatographic. View and download waters xbridge peptide beh c18 care and use manual online. What is the recommended storage solvent for the xbridge beh. Jan 15, 2021 use 100% acn when storing the xbridge beh amide or the acquity beh amide for longer than four 4 days. Xbridge oligonucleotide c18 columns waters corporation. An arrow on the column identification label indicates the correct direction of solvent flow. Column equilibration acquity uplc beh columns are shipped in 100% acetonitrile. Shipping discounts for multiple columns purchased at the same time. Waters xbridge peptide beh c18 a and 300a columns user. These columns provide good peak shape over a wide ph range 29 for additional method development flexibility with one family of columns. We have 1 waters xbridge peptide beh c18 manual available for free pdf download. Thank you for choosing a waters xbridge ost c18 column designed for lab scale.
Aluminum seal stoppers aluminum seals caps screw thread closed top. Uplc acquity beh rp18 column lifetime chromatography forum. Serving healthcare, research and clinical laboratories, and federal agencies. The embedded carbamate group in the bonded phase ligand provides alternate selectivity, especially for phenolic compounds compared to straight chain alkyl columns. Every column is tested and a performance test chromatogram along with a certification of acceptance are provided with each column. Care and use manual waters xbridge peptide beh c18 a and. Apr 14, 2020 what is the difference between xbridge c18, symmetry c18, and sunfire c18. Waters asr catalog waters massprep peptide mixture. The trifunctionally bonded c18 ligand provides you with a universal column for most hplc separations and gives you the widest. These columns are available in a broad range of sorbent particles and sizes. The attached manual describes the xbridge columns and includes user.
Anyone developing methods may prefer the speed offered by a less retentive column, while improving peak shape and chromatographic performance. This qualitative standard should be used on a regular basis for benchmarking column and system performance. Water equipment waters xbridge peptide beh c18 a and 300a columns user manual pdf download free 1. This standard is used to benchmark the acquity uplc and xbridge beh 300 and c18 as well as the acquity uplc csh and xselect column lines. New objective products deliver high sensitivity to proteomic analysis with picotip emitters, picofrit columns, and full sources for nano electrospray ionization mass spectrometry esi.
Beh columns as well as the identical xbridge are among the best i know concerning chemical stability. We providing 1 pdf manuals waters xbridge columns for download free by document types. The organic chcl 3 fractions were evaporated to dryness, reconstituted and injected onto a 150 x 2. This care and use manual describes about waters acquity premier glycan beh c18 ax column which is specifically developed to assist in the analysis of chemically labeled glycans that have been released from biopharmaceutical therapeutics. Manual filler manual filler accessories pipet filler controller pipets. What is the benefit of beh amide chemistry over beh hilic chemistry. The attached manual describes about xbridge peptide beh c18, a, and 300a columns and xbridge premier peptide beh c18, a columns and includes user recommendations. Where can i find the care and use instructions for a waters. The densely bonded and doubleendcapped cn provides increased column.
Xbridge beh shield rp18 columns complement the xbridge beh c18 and c8 phases and provide a necessary and powerful tool for hplc method development. Tips for increasing the life of sec columns wkb121506. Is it ok to backflush an acquity uplc beh c18 column. Waters asr catalog waters massprep oligonucleotide standard. Shorter chain bonded phases, such as cn can be less stable. Browse online or download water equipment waters xbridge columns user manual. Waters xbridge peptide beh c18 a and 300a columns user manual. The xbridge packing materials are manufactured in a cgmp, iso 9001. Getting started each xbridge oligonucleotide c 18 column is shipped with a certificate of analysis and a performance test chromatogram document. Determination of kerojet 8118 content in aviation turbine. In the list of user manuals, look for xselect csh xp 2. The trifunctionally bonded c18 ligand provides you with a universal column for most hplc separations and gives you the widest usable ph range, superior low ph stability, and ultralow column bleed. The trifunctionally bonded c18 ligand provides you with a universal column for most hplc separations and gives you the wides.
Waters xbridge peptide beh c18 prep column, a, 5um, 10mm x 100mm. May 15, 2020 for additional information, see xbridge columns care and use manual section v, column cleaning, regeneration. However, extra care must be taken to limit the water content in the m. The xbridge beh c8 sorbent is less retentive compared to the xbridge beh c18 sorbent. Both columns were kept at a temperature of 40 c in a hotdog xl5090 oven prolab. Kinesis chromatography and liquid handling consumables. Separation of nadolol racemates by high ph reversedphase.
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